2.1 Nature of Science

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Science uncertainty -exists because scientist can’t investigate things that can’t be seen.

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Hypothesis - possible explanation for event or set of observations.

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Changing science – mid – 1970’s technique for altering genes developed.

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Collaboration - scientists work in teams.

2.2 Science skills and methods

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Observing and questioning - can only study things that are seen.

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Observations - made directly with senses and indirectly with instruments to extend senses.

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Test hypothesis - carefully designed experiment.

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Variable - factor that can influence experiment.

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Experiment - designed to investigate effects of one variable.

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Experiment parts - control setup and experiment setup.

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Prediction - uses form of If…then statement.

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Sources for science - interviews, college and public libraries, experiments.

2.3 Tools of Biology

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Microscope - instrument that enlarges the image of specimen.

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Resolution - ability to see 2 points separately; sharper the image, better resolution.

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0.1mm - resolving power of unaided human eye.

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Light microscope - magnify images of 1500 times.

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Electron microscope - uses electrons instead of light; 1000 times better than light microscope.

2.4 Techniques in Biology

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   Field studies – studies that take place in organisms natural environment.

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Electrophoresis – molecules separated by shape, size and electrical charge.

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Cell Fractionation – study with large,pure samples of various organelles.

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Centrifuge – tool that separates mixtures by spinning them at various speeds.

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 Chromatography – technique to separate mixtures of liquid or gas

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Tissue culture – technique used to grow large amounts of a single kind of cell. It decreases the need for animal experiments.